Counting tranfected cells carrying betagal? Ideas and suggestions?
brukneri at ere.umontreal.ca
Sat Jun 15 14:34:01 EST 1996
I am trying to quantitate numbers of cells that pick up beta-gal in
a transfection experiment. I have tried to count groups of cells after
fixing and staining but I want better statistics... The numbers I am
counting could be potentially quite small (perhaps 1 in 10,000 to 100,000
Does anyone know of a company that cells petri dishes with gridlines
(smaller than the standard 1 cm X 1 cm phage plates) for counting cells
If not, how the hell are people counting there cells? I know you can
just make extracts of plates of cells and look at absorbance for instance
but that won`t help me. Also I know about facs sorting but that is a
little expensive for preliminary studies...
Thanks for any and all suggestions!
dellaire at odyssee.net
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