Snap Freezing: N2 or Isopentane?
100131.655 at CompuServe.COM
Sat Jun 15 10:59:33 EST 1996
Re: snap freezing tissue for RNA extraction--According to a
colleague, placing tissue in isopentane and then into liquid
nitrogen is more efficient than just putting it in a cryotube and
into liquid N2 because Iso-P is a liquid and 'immediately'
reaches -196, whereas the nitrogen forms an insulating layer of
gas around the tissue. Questions: (1) Is isopentane necessary
or desirable; and (2) does isopentane affect the quality of the
RNA subsequently extracted?
Dept. of Neuropathology
Institute of Psychiatry, London
More information about the Methods