PCR-dilema!

[Dr Richard Sallie]

Either works, but the short PCR (the extension is really a large PCR) is
cheaper and will be easier to manipuilate, providing the template you have
is fairly pure.  Good luck, Richard
In article <31C095DC.7843 at academ01.mty.itesm.mx>,
al701032 at MAIL.MTY.ITESM.MX (paola velasco) wrote:

> Hi, I need some advice, could you help me?
> 
> I have to amplify a fragment of 86 bp. I have two options: 1) Design 
> two primers of about 20-24 bases and run a PCR. or 2) Design two 
> oligonucleotides of 48 bp that overlap in 9 bases and do an extension 
> with a polymerase. Do you have any advices or experiences about this?
> 
>         Thanks,    Paola