Dr Richard Sallie
sallier at cygnus.uwa.edu.au
Sat Jun 15 20:54:36 EST 1996
Either works, but the short PCR (the extension is really a large PCR) is
cheaper and will be easier to manipuilate, providing the template you have
is fairly pure. Good luck, Richard
In article <31C095DC.7843 at academ01.mty.itesm.mx>,
al701032 at MAIL.MTY.ITESM.MX (paola velasco) wrote:
> Hi, I need some advice, could you help me?
> I have to amplify a fragment of 86 bp. I have two options: 1) Design
> two primers of about 20-24 bases and run a PCR. or 2) Design two
> oligonucleotides of 48 bp that overlap in 9 bases and do an extension
> with a polymerase. Do you have any advices or experiences about this?
> Thanks, Paola
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