PEG in ligations

Chris Boyd chrisb at hgu.mrc.ac.uk
Tue Jun 11 05:34:30 EST 1996


Michael W. Thompson (mthom0 at pop.uky.edu) wrote:
: 	    I agree, I have seldom needed to use PEG, even in tricky blunt-end ligations, and I've 
: also found that PEG strongly inhibits the subsequent transformation.

That's interesting.  I have found that the presence of PEG at and
somewhat above the levels one uses in ligation makes no difference to
transformation (by electroporation) frequencies.  

Best wishes,
--
Chris Boyd                       | from, | MRC Human Genetics Unit
chrisb at hgu.mrc.ac.uk             |  not  |  Western General Hospital
http://www.hgu.mrc.ac.uk/~chrisb |   for |   Edinburgh EH4 2XU, SCOTLAND



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