problem cloning a PCR amp. fragment

akhan at usa.pipeline.com akhan at usa.pipeline.com
Sat Jun 15 11:42:43 EST 1996


Hello.  I am trying to clone a 200bp PCR amplified fragment containing
EcoRI and BamHI sites in the primers (vector is pGEX).  I get positive
clones but none of them have inserts.  I have tried up to    1:10 ratio of
vector to insert.  Any ideas what is wrong?  Thanks in advance. 
Anwar Khan. 
akhan at unix1.sncc.lsu.edu 
  



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