problem cloning a PCR amp. fragment

Nick Jacobsen jacobsen at cf.ac.uk
Mon Jun 17 05:03:00 EST 1996


Hi Anwar,
        It sounds like you are putting enough insert in. You could try 
different ratios of vector to insert. You could also try treating the 
vector with calf intestinal alkaline phosphatase to dephos the ends. This 
at least will stop the vector religating to itself,

I hope this helps

Nick.




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