problem cloning a PCR amp. fragment

Nick Jacobsen jacobsen at
Mon Jun 17 05:03:00 EST 1996

Hi Anwar,
        It sounds like you are putting enough insert in. You could try 
different ratios of vector to insert. You could also try treating the 
vector with calf intestinal alkaline phosphatase to dephos the ends. This 
at least will stop the vector religating to itself,

I hope this helps


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