Acrylamide Gel setting too quickly -- IDEAS??
Mon Jun 17 09:49:45 EST 1996
Jerry Kropp wrote:
> Degas? Yuck!
> About 15yr ago some smartass from down the hall wandered in while I
> was cursing the water aspirator and asked "why are you degassing?
> That's what God made APS for."
> I use 60ul 25% APS for 30ml gel, and haven't degassed since. And I
> store the APS at -20 in 1ml aliquots. After ~10 freeze-thaw cycles I
> have to go to a fresh tube of the stuff. It keeps at least a yr at
> -20, unthawed.
> Hope this helps, Jerry
You can also store your aliquots at +4 degrees celsius and keep it there
for approx. 2 weeks. It works as well, contrary to literature.
u7g11at at lrz.uni-muenchen.de
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