cheap IPTG ?
Dr. Peter Gegenheimer
Tue Jun 18 01:07:02 EST 1996
In <4ppump$s16 at bubba.NMSU.Edu>, dkim at nmsu.edu (D. KIM) writes:
>This thread appeared a few months ago, and one of the solutions proposed
>for large-scale inductions was to use lactose as the inducer. Although
>it is metabolized by the bacteria, it is much cheaper than IPTG.
On a related note, for those who are using IPTG in agar plates to detect beta-gal produced from high-copy plasmids like pUC, pBS, etc.: the addition of IPTG is generally unnecessary unless the plasmid itself contains a functional lacI (repressor) gene.
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