cheap IPTG ?

Dr. Peter Gegenheimer RNAworld at
Tue Jun 18 01:07:02 EST 1996

In <4ppump$s16 at bubba.NMSU.Edu>, dkim at (D. KIM) writes:
>This thread appeared a few months ago, and one of the solutions proposed 
>for large-scale inductions was to use lactose as the inducer.  Although 
>it is metabolized by the bacteria, it is much cheaper than IPTG.
>Daniel Kim

On a related note, for those who are using IPTG in agar plates to detect beta-gal produced from high-copy plasmids like pUC, pBS, etc.: the addition of IPTG is generally unnecessary unless the plasmid itself contains a functional lacI (repressor) gene.

|  Dr. Peter Gegenheimer       |  Vox: 913-864-3939  FAX: 913-864-5321  |
|  Departments of Biochemistry |    pgegen at           |
|   and of Botany              |      |
|                              |                                        |
|  University of Kansas        |                                        |
|  2045 Haworth Hall           |  "The sleep of reason produces         |
|  Lawrence  KS  66045-2106    |   monsters."                  Goya     |

More information about the Methods mailing list