How to break out bacteria on the plate?

Marcel Hoffmann hoffmann at
Tue Jun 18 14:15:32 EST 1996

Jung Hoon Ahn wrote:
> I'm now finding the method to detect a enzyme activity on agar plate.
> It is intracellular enzyme and its activity can show halo on the plate.
> If we can break out our bacteria on the agar plate as it is,
> we can find colonies that have the enzyme.
> Is there any method or reagent to break out bacteria on the plate?

Hai you can try to place your petrie dish with the colonies 
upside down over a container with some chloroform in it.
*************  Dr. Marcel Hoffmann **************
************* Dept. of Pharmacochemistry ********
************* Vrije Universiteit ****************
************* Amsterdam  *********************

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