Brain Nuclear Extracts

[Marcel Hoffmann]

Sandra Pena De Ortiz wrote:
> 
> Hi:
> 
> Does anyone have a good protocol for preparation of nuclear extracts for
> gel shift assays?  Will really appreciate any help.  Thanks in advance.
> 
> Sandra Pena de Ortiz, Ph.D.
> 
> UTSA

Dear Sandra,

Good nuclear extracts for gel shifts assay's and in vitro 
transcriptions can be prepared according to the papers from 
Graves et al. Cell 44:565-576 1986 and Gorski et al. Cell 
47:767-776 1986.
I had good results with liver as well as spleen nuclear 
extracts prepared by this method.

Sincerely yours,

Dr. Marcel Hoffmann
Dept. of Pharmacochemistry
Vrije Universiteit
Amsterdam