Brain Nuclear Extracts
hoffmann at chem.vu.nl
Tue Jun 18 13:58:33 EST 1996
Sandra Pena De Ortiz wrote:
> Does anyone have a good protocol for preparation of nuclear extracts for
> gel shift assays? Will really appreciate any help. Thanks in advance.
> Sandra Pena de Ortiz, Ph.D.
Good nuclear extracts for gel shifts assay's and in vitro
transcriptions can be prepared according to the papers from
Graves et al. Cell 44:565-576 1986 and Gorski et al. Cell
I had good results with liver as well as spleen nuclear
extracts prepared by this method.
Dr. Marcel Hoffmann
Dept. of Pharmacochemistry
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