ANDREI.POPOV at bbsrc.ac.uk
Tue Jun 18 12:01:27 EST 1996
> I do not understand which reason is 95 C for 5 minutes for first
>cycle and 72 C for 10 minutes for last cycle
>Does any paper explain that ? Or somebody can explain that ? Thank a lot
No paper can explain this.
It is just another example of an illiterate approach;
however, PCR is robust enough to forgive sometimes even
more ridiculous cycles.
For more positive information have a look at:
More information about the Methods