pcr cycle

Andrei Popov ANDREI.POPOV at bbsrc.ac.uk
Tue Jun 18 12:01:27 EST 1996


> I do not understand which reason is   95 C for 5 minutes for first
>cycle  and 72 C for 10 minutes for last cycle
>Does any paper explain that ? Or somebody can explain that ? Thank a lot
>
>Shi-Jye Chu

No paper can explain this.
It is just another example of an illiterate approach;
however, PCR is robust enough to forgive sometimes even
more ridiculous cycles.
For more positive information have a look at:
PNAS 91:5695-
Gene 123:241-

Regards

Andrei





More information about the Methods mailing list