u9310871 at MUSS.CIS.MCMASTER.CA
Tue Jun 18 10:39:34 EST 1996
Hi! This is the guy who has been having problems with the MORPH
mutagenesis kit and the Stratagene Chameleon kit again. I've been able
to mini-prep my plasmid from the mut S bacteria for some unknown reason.
However, a new concern that has risen is that the efficiency of the
mutation is very low. Theoretically, one would expect approximately 50%
mutants. However, in my case, I got approximately 5 mutants out of 24
clones and of those, 4 produced some very strange results upon digestion
(The mutation was to create a new unique restriction endonuclease site).
Therefore, my efficiency was really only 1 out of 24 which is not
acceptable according to my supervisor. Any helpful comments would be
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