I need some advice on bandshift assays

[Andre Nantel]

In article <tmagee-1506961527490001 at max6-la-ca-43.earthlink.net>
tmagee at earthlink.net (Tom Magee) writes:

> Hi, I've been running standard band shift assays that are OK but not
> great.  I get large oval shaped  bands rather than sharp bands. I run a 20
> ul rxn.  Any suggestions as to getting sharp well defined bands?  Any
> foolproof protocols out there?  Thanks, Tom

You may also try to run your gels in a cold room. Helps a lot on
quality.

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Andre Nantel                             Purveyor of fine genetically-
Biotech Research Institute               engineered roadkills for the
National Research Council Canada         Information Superhighway
Montreal, Quebec
nantel at biotech.lan.nrc.ca or andre at bri.nrc.ca
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