chrisb at hgu.mrc.ac.uk
Wed Jun 12 11:11:10 EST 1996
Hans Dauwerse (dauw at ruly46.medfac.leidenuniv.nl) wrote:
: You could try strains as DH10b or DH5alphamcrSBCc, which...
[I assume you mean DH5alphamcrBC (Gibco Cat no. 18307-017)]
: ... should propagate plasmids with instable inserts.
No. These strains are likely to be no better than any other recA-
strains for tandem repeat instability, and certainly no better than
: Maybe yuo
: could consider to use a low copy plasmid.
A better idea, but still not _certain_ to resolve tandem repeat
instability. I don't know of _any_ strain where this problem has been
fully eradicated. There will always be sequences that cannot be cloned
stably in E. coli. It's a corollary of Godel's Theorem.
Chris Boyd | from, | MRC Human Genetics Unit
chrisb at hgu.mrc.ac.uk | not | Western General Hospital
http://www.hgu.mrc.ac.uk/~chrisb | for | Edinburgh EH4 2XU, SCOTLAND
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