mRNA for cDNA library

[Els Van Geldre]

Hi,

Several protocols for mRNA isolatation suggest the use of glycogen 
together with a salt in order to precipitate all the mRNA present in your 
sample. I' m wondering wether this glycogen doesn't have a bad influence 
on subsequent cDNA synthesis (this glycogen is namely clearly visible on 
gel). Furthermore I measure my mRNA concentration with 
UV-spectrophotometry. Could this have a influence or do I just stress too 
much ?

Thanks for answering,
Els Van Geldre