mRNA for cDNA library

Els Van Geldre Els.VanGeldre at rug.ac.be
Tue Jun 18 06:41:30 EST 1996


Hi,

Several protocols for mRNA isolatation suggest the use of glycogen 
together with a salt in order to precipitate all the mRNA present in your 
sample. I' m wondering wether this glycogen doesn't have a bad influence 
on subsequent cDNA synthesis (this glycogen is namely clearly visible on 
gel). Furthermore I measure my mRNA concentration with 
UV-spectrophotometry. Could this have a influence or do I just stress too 
much ?

Thanks for answering,
Els Van Geldre




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