check method of cell lysis

Jung Hoon Ahn jhahn at bioneer.kaist.ac.kr
Wed Jun 19 00:18:58 EST 1996


I want to check whether cell is lysed on agar plate.
I'm trying to clone a gene secreting a protein (ProA).
This protein (ProA) shows enzymatic activity on agar plate, 
although it is not secreted because there is no plamid of 
the secretion gene.

I have to firstly confirm whether it is from cell lysis or other.
Is there any method or reagent to check the cell lysis on agar plate.



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