Question about gel shift assay

Philippe Lefebvre plefebvre at pop.univ-lille2.fr
Wed Jun 19 10:12:46 EST 1996


Using shorter probes will help but in our experience, this will not allow you to get 
rid of non spec binding.  You 'd better try to use different non spec DNA such as 
salmon sperm DNA, calf thymus or poly dIdC.  Results can be really strikingly 
different!
Good luck.
Dr Phil Good




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