MORPH mutagenesis kit
u9310871 at MUSS.CIS.MCMASTER.CA
Wed Jun 19 12:30:08 EST 1996
Hi! This is the guy who was having trouble with the MORPH mutagenesis
kit. Well I've been able to isolate my plasmid from the mut S cells for
some unknown reason but the problem now has become one of efficiency.
Out of 24 tested clones, only 5 showed the creation of the mutation
(which was the creation of a unique restriction endonuclease site). Of
those 5, only 1 gave the expected results while the other 4 gave some
strange band sizes upon digestion and electrophoresis. My supervisor has
deemed that this type of efficiency (1 out of 24) is unacceptable since
if used to make mutants that don't necessarily create a new restriction
endonuclease site, screening will be a problem. I was wondering if
someone had any ideas why these results are so far off from the
theoretical efficiency of 50%. Any clues would be greatly appreciated.
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