Replication Origins and Two D gels... Question??
dellaire at ODYSSEE.NET
Wed Jun 19 19:12:46 EST 1996
I am thinking of looking at the replication status of a sequence near an
integrated vector in mouse cells. I want to know if compared to another
locus if the region is firing (replicating) at the same time or
differently (later or earlier... banding is not out but the two
sequences are perhaps in the same R band).
Can you look at origins in mammals by 2 D analysis? or do you need
something that fires many times in a cell cycle like polyoma...etc to be
able to see it on a 2-D gel?
Any suggestions or comments are welcome.
McGill Exp. Medicine
dellaire at odyssee.net
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