Replication Origins and Two D gels... Question??

[Graham Dellaire]

I am thinking of looking at the replication status of a sequence near an 
integrated vector in mouse cells.  I want to know if compared to another 
locus if the region is firing (replicating) at the same time or 
differently (later or earlier... banding is not out but the two 
sequences are perhaps in the same R band).

Can you look at origins in mammals by 2 D analysis? or do you need 
something that fires many times in a cell cycle like polyoma...etc to be 
able to see it on a 2-D gel?


Any suggestions or comments are welcome.


G. Dellaire
McGill Exp. Medicine
dellaire at odyssee.net