polytron for RNA extraction
s9033036 at COUGAR.VUT.EDU.AU
Wed Jun 19 18:01:14 EST 1996
Thanks Edouard for your advice. The polytron in our lab gets pretty=20
"messy" because another worker uses it to emulsify dried fruit samples.=20
It probably needs to be dismantled and soaked in something before using for=
Thanks also to the others who offered suggestions e.g. 1% SDS, RNase ZAP=20
or using denaturing solution such as guanidinium thiocyanate 6M or=20
Chomczynski's solution D. I might need to use them all.=20
Thanks again, Dianne
On 19 Jun 1996, Edouard Lauzier wrote:
> In article (Dans l'article)
> <Pine.SOL.3.91.960619080740.9707F-100000 at cougar>,
> s9033036 at COUGAR.VUT.EDU.AU (Dianne Emslie) wrote (=E9crivait)=A0:
> > Does anyone have a good method for cleaning a polytron homogeniser=20
> > suitable for RNA extraction of tissue? Some of its previous users were=
> > bit messy.
> > Cheers, Dianne
> Hello Diane...
> What do you mean by messy ?
> Whenever I use it, I first run it under NaOH 0.1N for 10 seconds at
> intensity 6 then wash it again in RNAse free water at the same
> parameters. That does it...
> Edouard Lauzier, B.Sc. elauzier at fse.ulaval.ca
> Physical Activity Science Laboratory
> (Laval University) G1K 7P4
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