nuclear run-on for low abundant RNA

[Olivier Gandrillon]

Hi

May I suggest that you can use a recently described technique, namely 
quantitative RT-PCR on the pre-messenget RNA (Elferink and Reiners 
(1996). BioTechniques vol 20 n°3, 470-477). If you succesfully use this 
technique, please let me know (I have'nt tried it personnaly but I would 
if I had to run-on a low expressed RNA.)

Olivier

Mari L. Shinohara wrote:
> 
> Dear netters,
> 
> I'm looking for methods of a nuclear run-on anaylsis for an RNA, which
> expresses a few % of total mRNA.  Do you know any good methods?  I
> heard that (I've never tried run-ons yet) this experiment can be really
> messy in terms of using a lot of 32P-UTP, which I want to avoid as much
> as I can...  Your idea would be greatly appreciated.
> 
> Mari L. Shinohara Ph. D.
> Dept. of Biochemistry
> Dartmouth Medical School