pcr cycle

Thibaud Le Mouel lemouel at antibes.inra.fr
Wed Jun 19 02:14:19 EST 1996


>Hi reader:
>
>    Recently I read some methods in the papers about the pcr cycles
>    the cycles is
>
>   95 C for 5 minutes, 60 C for 1 minute, 72 C for 1.5 minutes for one
>cycle
>    95 C for 1 minute, 60 C for 1 minute , 72 C for 1.5 minute for 26
>cycles
>     95 C for 1 minute, 60 C for i minute , 72 C for 10 minutes for one
>cycle
>  I do not understand which reason is   95 C for 5 minutes for first
>cycle  and 72 C for 10 minutes for last cycle
>Does any paper explain that ? Or somebody can explain that ? Thank a lot
>
>
>
>Shi-Jye Chu

Hi,

The first cycle is for a better denaturation and the last cycle is to do a
good elongation of all DNA.

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