NaI solution in geneclean
dkim at nmsu.edu
Tue Jun 18 11:52:59 EST 1996
In article <Pine.SOL.3.91.960617083844.29608A-100000 at lovelace.infobiogen.fr> Francois Coulier <coulier at infobiogen.fr> writes:
>I got the same problem with some batch of NaI. It did not affect the
>yield of purification, nor the clonabiliy of the purified fragment.
>interestingly, when I mixed the last few ml of the yellow NaI with a new
>batch, the color disappeared.
WRT yellow color in NaI solns.
You might see the FAQ entry about making your own Geneclean. One of the
details of making the NaI solution is to add sodium sulfite as a
preservative. This prevents yellowing, etc, I believe.
More information about the Methods