deletion mutant in Cyanobacteria

[Mikhail Alexeyev]

In article <31C666B6.42F5 at credit.erin.utoronto.ca>, Farid jalali
<fjalali at credit.erin.utoronto.ca> wrote:

> Hello,
> 
> I have designed a construct where a cloned cyanobacterial restriction 
> fragment will be cut, a region of the fragment removed, and in its place 
> a cassette conferring resistance to an antiobiotic will be placed. My 
> question deals with the fact that the region I will remove is not the 
> same size as the cassette. The species I work with undergoes homologous 
> recombination quite readily, and I am wondering if removing a region 
> from the construct that is larger than the genomic region is going to be 
> a problem. 
 
 
Don't worry, it should be just fine as long as you have sufficient regions
of homology on both sides of antibiotic-resistance cassette.

M. Alexeyev.









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