ckwen at expert.cc.purdue.edu
Wed Jun 19 10:23:13 EST 1996
Laurence Tiley (lst21 at cam.ac.uk) wrote:
: Paul has it right.
: Keeping the gel at -70 is necessary if you use preflashing, and for some of
: the fluors to work efficiently.
Just a follow up:
Keeping the film at -70C is to keep the e- at very low energy orbit, and
the perception of radiation may excite the low-energy e- to a higher energy
orbit. Following a back from higher-energy to low-energy orbit exchange, acc-
ording to E=Hv, light is emitted onto the film. At higher temperature, RT, the
e- is at higher energy orbit and much less excited light may be generated.
It is right that the S-35 does not need the intensifying screens. Please refer
to Manitas for more information.
More information about the Methods