DNA in ELISA?
s_mka1 at eduserv.its.unimelb.EDU.AU
Fri Jun 21 01:22:55 EST 1996
I'm wanting to perform ELISA's using DNA as the primary antigen. A
couple of the (very few) protocols I've come across pre-coat the wells
with protamine sulfate. I assume this is to enhance binding of the DNA
to the wells, but I've been able to find any other references to this use
of protamine sulfate. Also, can I assume that digesteable DNA is of a
purity that will give good results in an ELISA (ie. accessability to
primary antibody)? Does anyone have any experience or know of good
references dealing with using DNA in ELISA's?
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