Question about gel shift assay

[Heinz-Juergen Schaefers]

Philippe Lefebvre wrote:
> 
> Using shorter probes will help but in our experience, this will not allow you to get
> rid of non spec binding.  You 'd better try to use different non spec DNA such as
> salmon sperm DNA, calf thymus or poly dIdC.  Results can be really strikingly different!                                   ^^^^^^^^^^
HI!
Poly dIdC is absolutely necessary to get rid of non spec binding.... 
I have accidently done it once without: => terrible!!!!! 
Use 2 micrograms max (more does'nt make any further effect!)

Probe lenght: i use normaly very short probes; up to 25 b for a single transcription factor
site...                                  

If you want a complete protocol that works fine, pls E-mail me...

bye
	Heinz
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Dipl. Biol. Heinz-Juergen Schaefers
Universitaet Erlangen-Nuernberg / Med. Klinik IV / Nephrologisches Forschungslabor
Loschgestr. 8, D-91054 Erlangen, Germany
E-mail: dienstl.: mfm434 at rzmail.uni-erlangen.de / priv.: h-j.schaefers at erlangen.netsurf.de