Bacterial cDNA

Amie Franklin amief
Thu Jun 20 21:55:18 EST 1996


I have immunoscreened a cyanobacterial genomic library cloned into stratagene's
lambda ZAP (no affiliation). Protein was being produced in this case so I would
expect the two hybrid approach to work as well. However, there is one important
caveat when trying to express protein from cloned genomic DNA in these vectors.
Very often the protein is constitutively expressed off of its own promoter and
so you cannot discount positives when inducer (IPTG) is not present. 

good luck,
Amie

amief at candelab.berkeley.edu




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