cell energy metabolism and HPLC

Andreas Jordan jordan at ukrv.de
Thu Jun 20 06:26:51 EST 1996


Hello HPLC freaks !

Does anyone has a reference of a HPLC method for quantification of all 
relevant NTP,NDP, NMP and other energy metabolites from mammalian 
cells from one FN-biopsy sample ? What is the best method to harvest 
epithelial adherent cells to obtain a quite realistic cell energy 
status for further HPLC analysis ? What are alternative methods to 
quantify the cell energy status (except bioluminiscence) ?

Thanks so much in advance.



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