Begining with Baculovirus

Mr. M.J. Lush mlush at hgmp.mrc.ac.uk
Thu Jun 20 07:44:10 EST 1996


In article <jpl-180696150831 at mac-jpl.ibcp.fr>, jpl at ibcp.fr <jpl at ibcp.fr> wrote:
>
>	Hi! I will soon try to express the cDNA coding for my protein in a
>Baculovirus expression system. There are several kits and several vectors
>available to start such a job. The Novagen one is attractive according to
>is lower price. Let me know your experience with it or give me any valuable
>advice on how to begin with Baculovirus. Thanks for all.

	The girl on the next bench is working with Baculovirus...
the main problem turned out to be that you need to use a cell line
with a low passarge (Sp?) number.   This fact was not mentioned
in any of the protocols and only transpired from conversation 
with the tech support (eventually!).
-- 
Michael

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
NPC rights activist           |      Nameless Abominations are people too.



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