Please, help with RNA extraction.

gordon allison goa at
Thu Jun 20 06:20:28 EST 1996

If you get a cloudy interface with a milky top phase then after the 
centrifugation add an equal vol. of extraction buffer, mix and 
re-centrifuge. Sorry if this increases your vol. to precipitate but it 
works. If instead you have a very dense thick interface with a 
clear top phase, repeat the phenol extraction.

Best Luck,  Gordon.

More information about the Methods mailing list