x-gal plates for yeast

Przemko przemko at sgi.celgen.kuleuven.ac.be
Fri Jun 21 11:22:06 EST 1996


Hi!
Some years ago I remember I was growing yeast with LacZ constructs on 
plates and was looking for blue colonies. I remember it was an easy and 
quick way to identify various mutants that we were working on.
Now, I have to do something similar, but my yeast do not turn blue.

Does anyone have a working protocl for the x-gal in the plates? is there 
something fancy about the protocol? I have lost my old method folder and 
my memory is evidently failing me.

ThankX

Przemko



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