thawing & plating cell lines
leach at bu.edu
Fri Jun 21 11:08:19 EST 1996
What were the freezing conditions...in what media/temp etc..how long frozen?
cram at PIPELINE.COM wrote:
: my team and I are having trouble producing healthy-looking cell
: cultures at plating time. We thaw quickly, spin to remove DMSO and then
: plate. After one night, the cells are dividing slowly, and have a strange,
: small, sort of compact morphology. Overall, they are half the size as they
: appear when healthy. Only after another day or so do they have the nice
: flattened look to them, where you can see the cytoplasm and their nuclei
: are large, etc. Then they grow to confluency, no problem.
: Could this be due to a low density at plating, perhaps too low? Or is
: it more likely caused by our cryopreservation?
: Any input would be appreciated.
: Thank you in advance.
: Marc Rothman
: <cram at pipeline.com>
: Neurosurgery Research Labs
: Dept. of Neurosurgery
: NYU Medical Center
: 550 First Ave.
: NY, NY 10016
..... Martin Leach Email:leach at bu.edu
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