How do I label my PCR probe?

Jackie Song songj at aa.wl.com
Fri Jun 21 10:53:13 EST 1996


Hi!  I am trying to incorporate 32P-CTP into my PCR probe with no luck.  
I have been using my regular PCR protocol except with hot CTP and when I 
probe my Northern I get no results.  Does anyone have a proven method for 
this?  How hot should be probe be?  Is there any easy way for me to 
ensure the hot CTP is being incorporated into my product?	
Thanks.
Jackie



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