Sequencing from chromosomal DNA

Rafael Maldonado rafael at
Wed Jun 19 17:54:24 EST 1996

Hi netters,

Does anybody know if it is possible sequencing directly from bacterial 
choromosome preps? Do those cycle sequencing systems work in this case?

We have got the sequence of most of our gene, and we would like to 
sequence the rest without cloning it. There are only 160 bp missing!!

Any comments appreciated.


Rafael Maldonado                             | 'No te creas todo 
room 6160 Eccles Institute of Human Genetics |  lo que leas en
Department of Human Genetics		     |  internet.'
University of Utah			     |
Salt Lake City, Utah 84112. USA.	     |  "Don't believe  
Rafael.Maldonado at           |  everything you read
Rafael at	             |  in internet."
Tel: 801-581-4429			     |
Fax: 801-585-3910			     |	

More information about the Methods mailing list