Azidopine binding and pgp

Geri Grant ggrant at umabnet.ab.umd.edu
Fri Jun 21 10:43:25 EST 1996


Hi,

I have been trying to get a [3H]-azidopine binding assay to work on 
membranes isolated from cell lines, and have had no success.
I get clean 170 bands on western blots using C219, but when I try to bind 
the [3H]-azidopine and run a PAGE gel, I get no signal, just a wave of 
radioactivity at the base of my gel.

Has anyone any ideas on what I may be doing wrong?

Thanks in advance.
Geraldine.



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