Competent Sure E.coli?

H. ROYCHOWDHURY hroychow at NMSU.EDU
Fri Jun 21 17:03:07 EST 1996


On Fri, 21 Jun 1996, TJ Murphy wrote:

> Does anyone have a good method for making E.coli Sure strain (Stratagene) 
> cells competent for transformation by plasmids?
> 
> I'm looking for a frozen, chemical competent protocol, and would 
> appreciate recipes if you've got 'em.
> 
> Yes, I know you can buy them, but those prices make me ill!!!!
> 
> 
> Thanks in advance.
> 
> 

What about the Hanahan protocol for frozen competent cells? If you have a 
Red Book or Maniatis, you should find it, among others.

			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
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