How do I label my PCR probe?
ckwen at expert.cc.purdue.edu
Fri Jun 21 14:12:02 EST 1996
Jackie Song (songj at aa.wl.com) wrote:
: Hi! I am trying to incorporate 32P-CTP into my PCR probe with no luck.
: I have been using my regular PCR protocol except with hot CTP
By labeling DNA with P-32 using PCR, you should not use the regular PCR
protocol. The dNTP(dATP, dGTP, and dTTP) conc. should be decreased.
I have no problem to label the DNA generated from PCR or plasmid DNAs for
Southern and Northerns. You may use as little as 1-5ng of DNA as template
and get >10^9cpm/ug DNA.
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