How do I label my PCR probe?

Chi-kuang Wen ckwen at expert.cc.purdue.edu
Fri Jun 21 14:12:02 EST 1996


Jackie Song (songj at aa.wl.com) wrote:
: Hi!  I am trying to incorporate 32P-CTP into my PCR probe with no luck.  
: I have been using my regular PCR protocol except with hot CTP 

Dear Jacki:

By labeling DNA with P-32 using PCR, you should not use the regular PCR
protocol.  The dNTP(dATP, dGTP, and dTTP) conc. should be decreased.
I have no problem to label the DNA generated from PCR or plasmid DNAs for
Southern and Northerns.  You may use as little as 1-5ng of DNA as template
and get >10^9cpm/ug DNA.

regards,

Ck
.

 



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