How would you define a "cloning artifact"?

Dave Knorr dak at apldbio.com
Fri Jun 21 15:09:06 EST 1996


Okay, I'll bite.  A cloning artifact in DNA work is when a result in a
cloning attempt does not reflect the input template.  An example might be
cloning a PCR primer-dimer.  It's a real clone...you can sequence it...it
came out of the tube containing the input template, but it does not
reflect the population of fragments you were intending to get.  Other
examples might be rearrangments of sequence fragments, loss of
restriction sites, etc.



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