How do I label my PCR probe?
leach at bu.edu
Fri Jun 21 12:50:43 EST 1996
try labelling one of your pcr primers with gamma-32p-atp
use polynucleotide kinase to end label the oligo...
i prefer this method since it is less messy than using 32p-ctp - since this
will also label non-specific products (of all sizes.....creating a background
Jackie Song (songj at aa.wl.com) wrote:
: Hi! I am trying to incorporate 32P-CTP into my PCR probe with no luck.
: I have been using my regular PCR protocol except with hot CTP and when I
: probe my Northern I get no results. Does anyone have a proven method for
: this? How hot should be probe be? Is there any easy way for me to
: ensure the hot CTP is being incorporated into my product?
..... Martin Leach Email:leach at bu.edu
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