293 Cells: how to transfect?

iddavis at vms.cis.pitt.edu iddavis at vms.cis.pitt.edu
Fri Jun 21 14:00:10 EST 1996

In article <1996Jun21.142754 at opal.tufts.edu>, jstrassw at opal.tufts.edu writes:
> Hello!
> I am trying to use 293 cells because they are reported to be highly
> transfectable (>50% by Calcium Phosphate) and they are EGF-responsive.  The
> only problem is that the buggers dont adhere well to dishes.  I am loosing a
> lot of cells every time I wash after transfection.  Any suggestions?

Try using a milder detergent and don't use the scourer quite so much.
Also, 293 cells are notorious for not being suitable for dishwashers - perhaps
this is where your problem lies.

Seriously though, this shouldn't be a problem: centrifuge the pellet and
make sure you throw the right bit away!

Ian Davis					iddavis at vms.cis.pitt.edu

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