293 Cells: how to transfect?

jstrassw at opal.tufts.edu jstrassw at opal.tufts.edu
Fri Jun 21 14:27:54 EST 1996


Hello!
I am trying to use 293 cells because they are reported to be highly
transfectable (>50% by Calcium Phosphate) and they are EGF-responsive.  The
only problem is that the buggers dont adhere well to dishes.  I am loosing a
lot of cells every time I wash after transfection.  Any suggestions?

Thank you,
John






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