4 way ligations?

[Angel Yanagihara]

Hello...

I'm attempting a 4 way ligation and was wondering if anyone out
there has had any luck with this kind of thing.  I have built two
overlapping oligo stretches (about 60bps each) to link my insert
(about .8Kb) by bridging the 5' side of my insert to one side
of my vector and the other overlapping oligo is designed to
bridge the 3' side of my insert to the other open end of my
vector.  All sites involved are sticky end, unique, and compatible
only with each unique hookup involved.  I've tried to ligate
them all together in one ligation reaction, as well as attempting
various kinds of sequential ligations, with no success.

Any suggestions with this would be greatly appreciated

aloha and mahalo
Angel

angel at halia.pbrc.hawaii.edu