x-gal plates for yeast

Namy Olivier namy at lovelace.infobiogen.fr
Sat Jun 22 08:13:57 EST 1996


hello,
To screen blue colonies with x-gal plates It's better to use synthetic 
medium (see maniatis) than minimal medium. Don't forget to use 
phosphate buffer because x-gal doesn't work for yeast at the normal acidic 
pH of minimal medium; therefore a neutral pH medium is used (but many 
yeast strains will not not grow very well at this pH...)
Olivier Namy (France).



More information about the Methods mailing list