staining of living cells

Andy amcshea at
Sun Jun 23 14:45:08 EST 1996

Fritz Schwarzmann (Fritz.Schwarzmann at wrote:
: Dear Net-Users!

: I would be grateful to everyone who can give me a hint or send me a 
: protocol how to stain living and unfixed eucaryotic (lymphoid B-) cells for 
: expression of beta-galactosidase (as a marker for a recombinant 
: plasmid). The cells shall be used further for cloning of a cell line.

Wouldn't GFP be a whole lot easier? I never had much luck with great 
viability after b-gal staining in live cells.


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