x-gal plates for yeast
rkoedood at bio.bu.edu
Mon Jun 24 12:51:05 EST 1996
We do an agarose overlay with Phospahte buffer and X-Gal. Very easy, works
very well, and the yeast get to grow on whatever media they like best.
Just ask if you want more detail
Przemko (przemko at sgi.celgen.kuleuven.ac.be) wrote:
: Some years ago I remember I was growing yeast with LacZ constructs on
: plates and was looking for blue colonies. I remember it was an easy and
: quick way to identify various mutants that we were working on.
: Now, I have to do something similar, but my yeast do not turn blue.
: Does anyone have a working protocl for the x-gal in the plates? is there
: something fancy about the protocol? I have lost my old method folder and
: my memory is evidently failing me.
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