2D-PAGE and Clostridium

Michael Kertesz mkertesz at micro.biol.ethz.ch
Mon Jun 24 11:11:52 EST 1996


Hi everyone,
Has anybody out there done 2D-PAGE work with Clostridium species? I 
have found that the usual methods I use to solubilize the cell proteins 
don't work as well as for other bacterial species (though I've only used 
Gram-negs in the past). This may be in part because the cells are growing 
under nutrient-poor conditions, and tend to sporulate - unfortunately I 
can't get away from that, because it is part of the study. I'd be 
grateful for any tips from people who have solubilized total cell protein 
from Clostridia in the past.

many thanks,
 

Michael Kertesz

ETH-Microbiology,
ETH-Zentrum/LFV,
CH-8092 Zurich, Switzerland

tel: +41-1-632 3357
fax: +41-1-632 1148
e-mail: kertesz at micro.biol.ethz.ch



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