Frequency of BstE II cutting?

W R BENNETT bspwrb at bath.ac.uk
Mon Jun 24 10:24:45 EST 1996


OK,

If BstE II has a restriction site of GGTNACC, does it cut at the same
frequency as a six-cutter (i.e. an average 1 in 4096 disregarding sequence
distribution considerations), which is the "intuitive" answer, or does it
cut with reduced frequency (which is what I'd like!).  Promega's technical
department felt that it definitely cut at reduced frequency, but couldn't
really say why, or what the frequency was.... 

Thanks,

Bill Bennett
Developmental Biology Unit,
School of Biology and Biochemistry
University of Bath





More information about the Methods mailing list