293 Cells: how to transfect?
Dr Andrew J. Doherty
doherty at bsa.bristol.ac.uk
Mon Jun 24 03:03:20 EST 1996
HEK293 cells are notorius for not sticking to anything! We use them to
transfect with Glutamate receptor subunits and stick them to glass
coverslips coated with poly-D-lysine. They seem to be OK then. They can
be pathched, used in confocal microscopy and immunocytochemistry (live) -
so they will stick! But they are very sensitive to their environment.
They don't like being at room temperature when growing on ordinary tissue
culture plasticware and can die very easily if they don't like your
transfection methodology. We use a CaPO4 method, with overnight
transfection incubations, and they seem fine with this.
Hope this is helpful
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