Sequencing from chromosomal DNA
gkleiber at med.de
Mon Jun 24 16:37:53 EST 1996
Rafael Maldonado wrote:
> Hi netters,
> Does anybody know if it is possible sequencing directly from bacterial
> choromosome preps? Do those cycle sequencing systems work in this case?
> We have got the sequence of most of our gene, and we would like to
> sequence the rest without cloning it. There are only 160 bp missing!!
> Any comments appreciated.
Of course. Just needs DNA which is enough clear... usea commercial kit,
I´d recommend Quiagen or someting similar. Cycle sequencing and linear
sequencing both work. We do routinely Minipreps ans mentioned above, then
sequence them with the new ABI cycle seq. kit in a
one-cup-one-PCR-reaction. Works up to 800 bases.
Ask for more if you want.
> Rafael Maldonado | 'No te creas todo
> room 6160 Eccles Institute of Human Genetics | lo que leas en
> Department of Human Genetics | internet.'
> University of Utah |
> Salt Lake City, Utah 84112. USA. | "Don't believe
> Rafael.Maldonado at genetics.utah.edu | everything you read
> Rafael at howard.genetics.utah.edu | in internet."
> Tel: 801-581-4429 |
> Fax: 801-585-3910 |
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